上海開放生物科技有限公司
電 話：400-630-2280
郵 編：200233
Email：info@bio-ope.com
網 址：www.primedforprofits.com
Forget-Me-Not qPCR Master Mix
高性能、高性價比雙色反應跟蹤體系
購買500個反應包裝每個反應成本小于40美分
雙色反應體系幫助您避免失誤，節省時間、試劑、DNA模板樣品和成本
EvaGreen? 染料 – 靈敏度高于SYBR? Green, 更加安全和環保
Cheetah? 熱啟動反應酶 – 快速反應體系95°C兩分鐘即可完全激活
Forget-Me-Not?是一種高性能qPCR混合染料并且性價比明顯高于同類產品，正常情況每個反應只需40-55美分，相比于同類產品其它供應商每個反應0.5-1美元的成本。
Forget-Me-Not? qPCR Master Mix 是一種用于熒光定量PCR以及高分辨率熔解曲線分析的即用型試劑盒。Forget-Me-Not?結合低濃度的藍色染料, 加上具有高濃度藍色染料的DNA模板Buffer。當反應孔中加入 2X Forget-Me-Not? Master Mix 時，呈現淡藍色 (Figure 1, 左)。然后，加入帶有Buffer的DNA模板，呈現深藍色 (Figure 1, 右)。Forget-Me-Not? 能夠幫助您輕松識別是否忘加混合液還是模板從而避免由加樣而引起的失誤節省時間、試劑盒珍貴的DNA樣品。2X Forget-Me-Not? Master Mix 在沒有模板Buffer的時候也能夠正常使用。
Figure 1. Forget-Me-Not? allows you to see at a glance whether you’ve added reaction components to your tubes or wells. Left: 1X Forget-Me-Not? Master Mix; Right: Forget-Me-Not? Master Mix after addition of Forget-Me-Not? Template Buffer.
Forget-Me-Not? qPCR Master Mix
2X Master Mix 包含 EvaGreen?染料， Cheetah? 熱啟動 DNA 酶，dNTPs，和低濃度的嵌入藍色染料（可以用來區分空孔和已經加入反應混合液的孔）。 在Forget-Me-Not? Template Buffer有或者沒有的情況下Master Mix都可以使用。
Forget-Me-Not? 模板Buffer
The 40X Template Buffer 包含高濃度的藍色嵌入染料。將模板Buffer和DNA模板混合可以讓您在加樣時輕松跟蹤模板的加樣情況。
ROX 校正染料
Forget-Me-Not? qPCR Master Mix (貨號31041) 不包含 ROX 校正染料。如果您使用的儀器需要添加低濃度或者高濃度的 ROX 校正燃料，貨號31042產品中提供單獨的一瓶 ROX 校正染料和使用說明書。了解更多細節請下載Forget-Me-Not? qPCR Master Mix Protocol 。
EvaGreen? 染料
EvaGreen?染料是Biotium公司研發的新一代DNA結合染料，它同時適合于實時定量PCR以及高分辨率熔解曲線分析（HRM）。這種染料通過一種被稱為“按要求釋放”的全新機制，選擇性結合雙鏈DNA，從而大大降低了染料對PCR反應的抑制作用。EvaGreen?染料的另一個重要的特性是安全、無毒。目前商業化的核酸染料大都會誘導基因的突變，而且很少做過完整的毒性研究，這對使用者的健康存在著巨大的潛在危害。比如在PCR實驗中常用的SYBR? Green I染料，有文獻報道其比EB具有更大的環境毒性(Ohta, et al. Mutation Research, 492,91-97(2001)).，雖然SYBR? Green I 本身誘變性很弱，但由于它可能在活細胞中抑制正常DNA的修復機制，使其有誘變增強的作用?？紤]到PCR的廣泛使用，核酸染料的安全性應該被足夠重視，基于以上的理念，Biotium的科學家研發出了新一代的核酸染料EvaGreen?。EvaGreen? 由于降低了細胞膜穿透性，因而比SYBR? Green I更加安全（圖-2）。獨立實驗室的測試結果顯示，EvaGreen? 既沒有誘變性也沒有細胞毒性，廢棄物可直接倒入下水 道。完整的安全性報告請下載 EvaGreen? Dye Safety Report。
基于EvaGreen?染料的Master mix另一個好處就是當您需要用凝膠電泳分析PCR產物時無需再添加染料。EvaGreen? 染料起到了DNA預染色的效果，電泳后可以直接觀察結果。
Cheetah? 熱啟動PCR酶
Cheetah? 化學修飾熱啟動酶是 Biotium的專利產品，由于使用了創新的修飾試劑品質得以提升，在室溫條件下完全抑制酶活性， 95°C只需兩分鐘即可完全激活,，非常適合快速PCR反應體系。
Figure 2. Comparison of GAPDH fast-cycling real time qPCR assay using Forget-Me-Not? qPCR Master Mix, Biotium’s Fast EvaGreen? qPCR Master Mix, or Qiagen’s QuantiNova? SYBR? Green qPCR Master Mix. A. Raw fluorescence data. EvaGreen?-based master mixes yield higher fluorescence signal compared to the SYBR? Green-based master mix. B. Data plotted on logarithmic scale. Forget-Me-Not? performs as well or better than other PCR master mixes.
PCR性能穩定
Forget-Me-Not? qPCR Master Mix 適用于快速PCR反應體系，在正常的反應體系中也表現出優異的性能。Forget-Me-Not? qPCR Master Mix 性能和我們的Fast EvaGreen? Master Mix同樣優秀，和 Qiagen的 QuantiNova? SYBR? Green PCR Master Mix一樣優秀甚至更穩定靈敏 (Figure 2)，尤其能夠讓您輕松確保每次加樣的精準。
相關Biotium的產品Fast EvaGreen?, Fast Plus EvaGreen?, and Fast Probe qPCR Master Mixes.
| Product Name | Catalog Number | Features |
|---|---|---|
| Forget-Me-Not qPCR Master Mix | 31041 | EvaGreen dye-based master mix with a unique two-color system for tracking which reagents you've added to your reaction. |
| Forget-Me-Not qPCR Master Mix with ROX | 31042 | EvaGreen dye-based master mix with a unique two-color system for tracking which reagents you've added to your reaction, with ROX reference dye. |
| Fast EvaGreen Master Mix | 31003 | EvaGreen dye-based master mix, recommended for most qPCR applications. Supplied with separate vial of ROX reference dye, for use with either high-ROX or low-ROX instruments. |
| Fast Plus EvaGreen qPCR Master Mix | 31020 | EvaGreen dye-based maser mix, optimized for challenging targets. No ROX, for use with: BioRad: iCycler, MyiQ, MiQ 2, iQ 5, CFX-96, CFX-384, MJ Opticon, Option2, Chromo4, MiniOpticon Qiagen: Rotor-Gene Q, Rotor-Gene3000, Rotor-Gene 6000 Eppendorf: Mastercycler realplex Illumina: Eco RealTime PCR System Cepheid: SmartCyler Roche: LightCycler 480, LightCycler 2.0 |
| Fast Plus EvaGreen qPCR Master Mix, Low ROX | 31014 | EvaGreen dye based maser mix, optimized for challenging targets. Low ROX for use with: ABI: 7500, 7500 Fast, ViiA 7 Stratagene: MX4000P, MX3000P, MX3005P |
| Fast Plus EvaGreen qPCR Master Mix, High ROX | 31015 | EvaGreen dye based maser mix, optimized for challenging targets. High ROX, for use with: ABI: 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne plus |
| Fast Probe Master Mix, no ROX | 31005 | Master mix for use with oligo-based probes. No ROX, for use with: BioRad: iCycler, MyiQ, MiQ 2, iQ 5, CFX-96, CFX-384, MJ Opticon, Option2, Chromo4, MiniOpticon Qiagen: Roto-Gene Q, Roto-Gene3000, Roto-Gene 6000 Eppendorf: Mastercycler realplex Illumina: Eco RealTime PCR System Cepheid: SmartCyler Roche: LIghtCycler 480, LightCycler 2.0 |
| Fast Probe Master Mix, with ROX | 31016 | Master mix for use with oligo-based probes. With ROX, for use with: ABI: 7500, 7500 Fast, 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne plus Stratagene: MX4000P, MX3000P, MX3005P |
| EvaGreen Dye, 20X in water | 31000 | Stand-alone EvaGreen dye for use in your own qPCR reaction mix. |
| EvaGreen Dye, 2000X in DMSO | 31019 | Concentrated stand-alone EvaGreen dye for specialized applications. |
| Cheetah HotStart Taq Polymerase | 29050 | Stand-alone fast-activating Cheetah Taq for hot-start PCR. |
EvaGreen? Dye and Cheetah? HotStart Taq DNA Polymerase are covered under US and international patents. QuantiNova? is a registered trademark of Qiagen Group. SYBR? Green is a registered trademark of Molecular Probes, Inc./Life Technologies. *HRM? is a registered trademark of Idaho Technologies, Inc./BioFire Defense, LLC and its use may require a license.
