CF™568 Dye

CF™568 is a red fluorescent dye with an excitation spectrum optimally matching the 568 nm line of the Ar-Kr mixed-gas laser (Figure 1). Antibody conjugates of the dye are much brighter than those of Alexa Fluor® 568. In addition, the photostability of CF™568 is superior to that of Alexa Fluor® 568, making CF™568 a much better choice for demanding applications, such as confocal and single molecule imaging.

A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and α-bungarotoxin are available for CF™ dyes.

Figure 1. Absorption and emission spectra of CF™568 conjugated to goat anti-mouse IgG in PBS.

 

Figure 2. Jurkat cells were stained with intracellular CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD FACS Calibur in the FL2 channel. The bars represent the relative fluorescence of the geometric means of the population of cells.

 

Figure 3. Jurkat cells were stained with intracellular biotin-CD3 followed by streptavidin CF568 or Alexa Fluor568 conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image.