One-Step Blue? is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence. In addition to rapid results and simple staining, One-Step Blue? offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid.
One-Step Blue? has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing.
Biotium also offers One-Step Lumitein? Protein Gel Stain, a rapid, easy-to-use, non-toxic red fluorescent protein gel stain for detection using a UV transilluminator or laser gel scanner, that costs significantly less than other fluorescent protein gels stains.